I-Infectious Bovine Rhinotracheitis Virus Antibody ELISA Detection Kit

Imodeli ehlobene ye- " VD105E
Abbr.:IBRV Ab Elisa
Isibonelo: i-serum

Incazelo

Ukucaciswa

BVDV Ab Elisa

Ukusetshenziswa Okuhlosiwe: EsithathelwanayoI-Bovine Rhinotracheitis VirusI-antibody ELISA Detection Kit isetshenziselwaukunquma i-rhinotracheitis esithathelwanayoamasosha omzimba ku-bovine serum in vitro.

Isifanekiso: serum

Izinto Ezinikeziwe:

Infectious Bovine Rhinotracheitis Virus Antibody ELISA Detection Kit

Izinqubo Zokuhlola Nokuhumusha

ISINYATHELO 1 Inqubo Yokuhlola

1.1 Ukulungiselela Isixazululo Sokugeza

Wukuhlushwa kwe-orking wi-ashing liquid:Ngaphambi kokusetshenziswa, isisombululo sokugeza kufanele sibuyiselwe ekamelweni lokushisa (20-25 degrees) futhi sinyakaziswe ngokulinganayo ukuze senze okufanayo. Bese uhlanza izikhathi ezingu-20 isisombululo sokugeza esigxiliwe ngamanzi ahlanzekile (isibonelo, isixazululo sokugeza esigxilile esingu-5ml sengezwa ku-95ml amanzi ahlanzekile), hlanganisa kahle, futhi uketshezi lokugeza oluhlanjululwe lungagcinwa ku-2-8℃izinsuku ezingu-7.

1.2 Ukulungiselela Isampula

① Igazi lesilwane lonke lathathwa, lafakwa i-centrifused ku-4000rpm imizuzu engu-10, futhi kwaqoqwa amandla amakhulu. I-Serum ingabuye ihlanganiswe ngokwemvelo ngaphandle kwe-hemolysis.

② Nciphisa i-serum okufanele ihlolwe izikhathi ezingu-50 kupuleti lokuhlanjululwa kwe-serum (isibonelo: engeza i-3ul ye-serum ezohlolwa ku-147ul yesampula ye-diluent).

Qaphela: Izilawuli ezingezinhle nezihle akumele zihlanjululwe

1.3 Inqubo Yokuhlola

①Thatha ilebula le-enzyme i-Microplate (ngokwenani lamasampuli, ingahlakazwa futhi isetshenziswe izikhathi eziningana), engeza i-100ul ye-serum ehlanjululwe kulebula ye-enzyme ye-Microplate, setha imithombo emibili ngayinye yokulawula okungalungile nokulawula okuhle, bese wengeza i-100ul ekuphenduleni kahle ngokulandelanayo. Gcoba ngobumnene isampula emthonjeni, usebenzisa ifilimu yepuleti lokuvala ukuvala ipuleti, bese ufukamela ku-37℃imizuzu engu-30.

②Vula ifilimu yepuleti lokuvala, vuthulula isixazululo epuletini kahle, engeza u-300µl woketshezi lokuwasha lokugxilisa okusebenzayo emthonjeni ngamunye, bese ulahla uketshezi emthonjeni. Phinda lezi zinyathelo ezingenhla izikhathi ezi-5, bese ekugcineni ubambe ephepheni elihlanzekile elimuncayo.

③I-conjugate ye-enzyme ehlanjululwe: Nciphisa i-enzyme conjugate (80×) izikhathi ezingu-80 (isibonelo: i-10ul enzyme conjugate concentrate + 790i-ul sample diluent, inani elithile elidingekayo libalwa ngokuvumelana nenani lamasampuli okuhlola), hlanganisa kahle futhi ubeke eceleni.

④Engeza u-100µl we-Enzyme Conjugate emthonjeni ngamunye, vala ipuleti, futhi ufukamele ku-37℃imizuzu engu-30.

⑤Ukugeza ipuleti le-enzyme, ukusebenza okuqondile kuyefana nalokhu okungenhla ②.

⑥Engeza isixazululo se-substrate esingu-100µl emthonjeni ngamunye, vala ipuleti bese ufukamela imizuzu engu-10 nge-37℃kude nokukhanya.

ISINYATHELO 2 Ukuhunyushwa komphumela

Inani le-OD450 lomthombo ngamunye lafundwa kumfundi we-microplate we-96-well.

2.1 Ukuqinisekisa Isilinganiso senani le-OD450nm lokulawula okunegethivu lalingu-< 0.25 futhi isilinganiso senani le-OD450nm lokulawula okuphozithivu LaliKhulu noma lilingana no-0.6, imiphumela yokuhlolwa ibivumelekile. Uma kungenjalo, ukuhlolwa kufanele kuphindwe.

2.2 Indlela yokubala

Kusampula ngayinye, bala i-S/P ngendlela elandelayo usebenzisa isampula elilungisiwe kanye namanani okulawula:

product-554-49

2.3 Ukuhunyushwa Kwemiphumela

Uma i-S/P <0.2, ibhekwa njengenegethivu.

Uma i-S/P > 0.3, ibhekwa njengephozithivu.

Lapho 0.2 Ngaphansi noma ilingana no-S/P Ngaphansi noma ilingana no-0.3, kwahlulelwa njengokusolisayo, futhi kusikisela ukuphinda ukusampula nokuhlolwa ngemva kwesonto elilodwa.

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